HumanTLR5 Stable Reporter Cell Line
| Name | HumanTLR5 Stable Reporter Cell Line |
|---|---|
| Supplier | Novus Biologicals |
| Catalog | NBP2-26277 |
| Category | Cell Line |
| Prices | $1,205.00 |
| Sizes | 1 vial |
| Applications | FC |
| Species | Human |
| Growth Mode | Adherent Morphology: Epithelial |
| Description | The TLR5 reporter line can be used for TLR5-dependent functional assays as well as screening of TLR5 agonists or antagonists. The TLR5 reporter cell line has been validated by flow cytometry (fig. 1) and ligand dose response assay (fig. 2). Contents: 3~4 x 10^6 cells Biosafety Level: 2 |
| Supplier Page | Shop |
Product images
![Flow Cytometry: Human TLR5 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26277] - Surface staining of TLR5 on the TLR5 reporter cell line was analyzed by flow cytometry using a PE-conjugated TLR5 antibody. Flow samples were prepared using TLR Staining Flow Kit. Purple: Cells without antibody; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1 ug anti-TLR5-PE; Red: TLR5 reporter cell line stained with 1 ug anti-TLR5-PE.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_1/19729_Human-TLR5-NF-kB-SEAP---%2528SEAPorter%2528TM%2529%2529-Stable-Reporter-Cell-Line-Flow-Cytometry-NBP2-26277-img0005.jpg)
Flow Cytometry: Human TLR5 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26277] - Surface staining of TLR5 on the TLR5 reporter cell line was analyzed by flow cytometry using a PE-conjugated TLR5 antibody. Flow samples were prepared using TLR Staining Flow Kit. Purple: Cells without antibody; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1 ug anti-TLR5-PE; Red: TLR5 reporter cell line stained with 1 ug anti-TLR5-PE.
![Ligand Activation: Human TLR5 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26277] - TLR5/NF-kB SEAPorter™ HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of flagellin for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. Flagellin-mediated TLR5 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of flagellin. The EC50, or the half maximal activity concentration, represents the concentration of flagellin that was required for 50% activation of TLR5 as measured by SEAP activity.Data Summary: Flagellin activated the cell line in a dose response manner, of which EC50 was measured as 0.29 ng/ml.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_1/19730_Human-TLR5-NF-kB-SEAP---%2528SEAPorter%2528TM%2529%2529-Stable-Reporter-Cell-Line-Ligand-Activation-NBP2-26277-img0006.jpg)
Ligand Activation: Human TLR5 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26277] - TLR5/NF-kB SEAPorter™ HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of flagellin for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. Flagellin-mediated TLR5 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of flagellin. The EC50, or the half maximal activity concentration, represents the concentration of flagellin that was required for 50% activation of TLR5 as measured by SEAP activity.Data Summary: Flagellin activated the cell line in a dose response manner, of which EC50 was measured as 0.29 ng/ml.
Product References
Identification of substituted pyrimido[5,4-b]indoles as selective Toll-like - Identification of substituted pyrimido[5,4-b]indoles as selective Toll-like
Chan M, Hayashi T, Mathewson RD, Nour A, Hayashi Y, Yao S, Tawatao RI, Crain B, Tsigelny IF, Kouznetsova VL, Messer K, Pu M, Corr M, Carson DA, Cottam HB. J Med Chem. 2013 Jun 13;56(11):4206-23.
Role of TLR5 and flagella in bacillus intraocular infection. - Role of TLR5 and flagella in bacillus intraocular infection.
Parkunan SM, Astley R, Callegan MC. PLoS One. 2014 Jun 24;9(6):e100543.